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首页> 外文期刊>Journal of Immunological Methods >Rapid single-step methods for detection of two immune defence gene polymorphisms: the myeloperoxidase (MPO) G-129A and the Fc gamma receptor 2A (FCGR2A) H/R131.
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Rapid single-step methods for detection of two immune defence gene polymorphisms: the myeloperoxidase (MPO) G-129A and the Fc gamma receptor 2A (FCGR2A) H/R131.

机译:快速的一步法检测两个免疫防御基因多态性:髓过氧化物酶(MPO)G-129A和Fcγ受体2A(FCGR2A)H / R131。

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摘要

Polymorphisms of immune defence genes may act as disease modifiers and are studied by many researchers. A conclusive analysis of the impact of genetic variations typically requires a large number of sample specimens, and in retrospective studies this may include samples of reduced quality, e.g. formalin-fixed paraffin-embedded tissue specimens. Here we describe two new single-step methods for rapid and sensitive analysis of: 1. The G-129A myeloperoxidase (MPO) promoter polymorphism, which affects the amount of myeloperoxidase in neutrophils. 2. The Fc gamma receptor 2A (FCGR2A)-H/R131 polymorphism, which is critical to the binding of IgG2 immune complexes to phagocytes.
机译:免疫防御基因的多态性可能是疾病的修饰剂,许多研究者对此进行了研究。对遗传变异影响的结论性分析通常需要大量样本,在回顾性研究中,这可能包括质量降低的样本,例如福尔马林固定石蜡包埋的组织标本。在这里,我们描述了两种新的用于快速,灵敏分析的单步方法:1. G-129A髓过氧化物酶(MPO)启动子多态性,它影响嗜中性粒细胞中髓过氧化物酶的量。 2. Fcγ受体2A(FCGR2A)-H / R131多态性,对IgG2免疫复合物与吞噬细胞的结合至关重要。

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