Using elemental ratios of calcium and strontium to track calcium availability in the freshwater zooplankton Daphnia pulicaria

摘要

The assimilation of elements by an organism is dependent on both the environmental availability of the element and the processes of the organism. For some elements, organisms have a challenging time discriminating between nearly identical chemical analogs, for example, calcium and strontium. We tested the hypothesis that in environments where a desired element is scarce, the organism will assimilate a chemically similar analog at an increased rate. Populations of Daphnia pulicaria were manipulated using a microcosm in situ experiment and the results of that experiment tested with a field survey. Experimental results indicated a correlation between higher environmental calcium concentration and lower [Sr]/[Ca] ratios (R-2 = 0.91, p < 0.05), suggesting that Daphnia in high calcium environments will assimilate more calcium relative to strontium. Field survey results across eight lakes confirmed that as lake calcium concentration increased, the value of [Sr]/[Ca] between the organism and the lake water decreased (R-2 = 0.60, p < 0.05). Measurement of the elemental ratio of major and trace element analogs within organisms compared to their environments may be a useful tool for measuring the relative bioavailability of the major element, and provide insight into elemental limitation in other calcifying aquatic invertebrates.