Effect of angiotensin II on voltage-gated sodium currents in aortic baroreceptor neurons and arterial baroreflex sensitivity in heart failure rats

摘要

Background:Impairment of arterial baroreflex sensitivity is associated with mortality in patients with chronic heart failure (CHF). Elevation of plasma angiotension II (Ang II) contributes to arterial baroreflex dysfunction in CHF. A reduced number of voltage-gated sodium (Na-v) channels in aortic baroreceptor neurons are involved in CHF-blunted arterial baroreflex.Method:In this study, we investigated acute effect of Ang II on Na-v currents in the aortic baroreceptor neuron and on arterial baroreflex in sham and coronary artery ligation-induced CHF rats.Results:Using Ang II I-125 radioimmunoassay, real-time reverse transcription-PCR and western blot, we found that Ang II levels, and mRNA and protein expression of angiotension II type 1 receptor in nodose ganglia from CHF rats were higher than that from sham rats. Local microinjection of Ang II (0.2nmol) into the nodose ganglia decreased the arterial baroreflex sensitivity in sham rats, whereas losartan (1nmol, an angiotension II type 1 receptor antagonist) improved the arterial baroreflex sensitivity in CHF rats. Data from patch-clamp recording showed that Ang II (100nmol/l) acutely inhibited Na-v currents in the aortic baroreceptor neurons from sham and CHF rats. In particular, inhibitory effect of Ang II on Na-v currents in the aortic baroreceptor neurons was larger in CHF rats than that in sham rats. Losartan (1mol/l) totally abolished the inhibitory effect of Ang II on Na-v currents in sham and CHF aortic baroreceptor neurons.Conclusion:These results suggest that elevation of endogenous Ang II in the nodose ganglia contributes to impairment of the arterial baroreflex function in CHF rats through inhibiting Na-v channels.