INFLUENCE OF ETHYLENEDIAMINE-N,N′-DISUCCINIC ACID (EDDS) CONCENTRATION ON THE BACTERICIDAL ACTIVITY OF FATTY ACIDS IN VITRO

摘要

The antibacterial activity of mixtures of ethylenediamine-N,N′-disuccinic acid (EDDS) and antibacterial fatty acids (FA) was examined using the agar diffusion assay. Solutions of caproic, caprylic, capric and lauric acids dissolved in potassium hydroxide (KOH) were supplemented with 0, 5 or 10 mM of EDDS and adjusted to pH 11.0 with citric acid. Wells made in agar media seeded with one of nine bacterial isolates were filled with FA-KOH or FA-KOH-EDDS solutions. After incubation of agar plates, zonesof inhibition of bacterial growth around the agar wells were measured. Results indicated that caproic-KOH-EDDS and caprylic-KOH-EDDS inhibited growth of more bacterial isolates than caproic-KOH and caprylic-KOH, respectively. Although, capric-KOH and lauric-KOH produced zones of inhibition of all nine bacterial isolates, significantly (P ≤ 0.05) larger zones of inhibition were produced by capric-KOH-EDDS and lauric-KOH-EDDS. Findings of this study indicate that mixtures of FA-KOH-EDDS possess greaterantibacterial activity than FA-KOH.