Alterations in iron, zinc and major plasma proteins of rainbow trout, Oncorhynchus mykiss (Walbaum), and brook trout, Salvelinus fontinalis (Mitchill), with sterile peritonitis induced by oil-adjuvanted multivalent bacterin vaccination

摘要

Inflammation was induced by i.p. lipogen triple vaccination to rainbow trout and brook trout to study the early humoral response. Severe acute sterile peritonitis occurred after vaccination in both species. Both species also had a rapid transient reduction in plasma iron concentration 3 days after vaccination. Moderate hypoferraemia persisted to day 14 in brook trout, but returned to normal by day 7 in rainbow trout. Plasma zinc decreased sharply 3 days after vaccination in rainbow trout and returned nearly to control levels by day 10; however, plasma zinc did not change in brook trout. Two-dimensional sodium dodecyl sulfate polyacrylamide electrophoresis of plasma proteins revealed that increased amounts of a 48-kDa protein group coincided with the hypoferraemic response in rainbow trout. In addition, a modest elevation in a 16-kDa protein group also occurred in rainbow trout. It is concluded that the rapid changes in plasma iron in both species and mild elevation of two putative acute phase plasma proteins associated with vaccine-induced inflammation in rainbow trout.