Real-time PCR identification of lake whitefish Coregonus clupeaformis in the Laurentian Great Lakes

摘要

The purpose of this study was to develop a real-time PCR assay to specifically identify lake whitefish Coregonus clupeaformis in larval fish assemblages based on a 122bp amplicon from the mitochondrial genome. The efficiency of the reaction, as calculated from the standard curve, was 9077% with the standard curve having an r(2) value of 0998. Specificity of the assay provided single melt peak in a melt-curve analysis and amplification of only the target species. The assay successfully identified target DNA in as low as 01% proportion of a DNA mixture. This assay was designed on the portable Smart Cycler II platform and can be used in both field and laboratory settings to successfully identify C. clupeaformis. (C) 2016 The Fisheries Society of the British Isles