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Characterization of mosquito larvicidal parasporal inclusions of aBacillus thuringiensis serovar higo strain

机译:苏云金芽孢杆菌致病性高致病菌株的蚊子幼虫孢子囊夹杂物的表征

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摘要

The parasporal inclusion proteins of the type strain of Bacillus thuringiensis serovar higo (H44), that have moderate mosquitocidal activity, were characterized. The purified parasporal inclusions, spherical in shape, were examined for activity against the two mosquito species, Culex pipiens molestus and Anopheles stephensi and the moth-fly, Telmatoscopus albipunctatus. The LC50 values of the inclusion for the two mosquitoes were 3.41 and 0.15 mu g ml(-1), respectively. No mortality was shown for T. albipunctatus larvae by the inclusions at concentrations up to 1 mg ml(-1). Solubilized parasporal inclusions exhibited no haemolytic activity against sheep erythrocytes. Parasporal inclusions consisted of eight proteins with molecular masses of 98, 91, 71, 63, 59, 50, 44 and 27 kDa. Of these, the 50 and 44 kDa proteins were the major components. Analysis with immunoblotting revealed that, among several inclusion proteins of B. thuringiensis serovar israelensis, only two proteins of 130 kDa and 110 kDa reacted weakly with antibodies against higo proteins. N-terminal amino acid sequences of the 98, 91, and 71 kDa proteins showed 85-100% identity to those of the two established Cry protein classes, Cry4A and Cry10A.
机译:鉴定了具有中等灭蚊活性的苏云金芽孢杆菌血清高致病性(H44)型菌株的孢子旁包合蛋白。检查了纯化的球形孢子囊夹杂物对两种蚊虫(淡色库蚊和斯蒂芬按蚊)和飞蛾Telmatoscopus albipunctatus的活性。两种蚊子的包裹体的LC50值分别为3.41和0.15μg ml(-1)。最高浓度为1 mg ml(-1)的夹杂物未显示出对白线虫幼虫的死亡率。溶解的孢子旁夹杂物对绵羊红细胞没有溶血活性。孢子旁包涵体由八种蛋白质组成,分子量分别为98、91、71、63、59、50、44和27 kDa。其中,50和44 kDa蛋白是主要成分。免疫印迹分析表明,在苏云金芽孢杆菌血清型以色列几种包涵体蛋白中,只有130 kDa和110 kDa的两种蛋白与针对higo蛋白的抗体反应较弱。 98、91和71 kDa蛋白的N末端氨基酸序列与两个已建立的Cry蛋白类别Cry4A和Cry10A具有85-100%的同一性。

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