Preventive effects of an original combination of grape seed polyphenols with amine fluoride on dental biofilm formation and oxidative damage by oral bacteria.

摘要

Aims: To investigate the preventive effects of an original combination of a grape seed extract (GSE) with an amine fluoride (FluorinolReg.) on dental plaque formation and oxidative damage caused by oral bacteria. Methods and Results: The antibacterial activity of the compounds was assessed using the broth macrodilution method, and their antiplaque activity was evaluated on a multispecies biofilm grown on saliva-coated hydroxyapatite discs. The effect on glucosyltransferases activity was analysed through reductions in the overall reaction and the quantity of insoluble glucan synthesized. The combination of 2000 micro g ml-1 of GSE with 10.2 mg ml-1 of FluorinolReg. significantly decreased the biofilm formation (up to 4.76 log₁₀ of reduction) and inhibited by 97.4% the insoluble glucan synthesis by glucosyltransferases. The antioxidant activity of this combination, alone or incorporated into a formulated mouthwash (Eludril dailyReg.), was determined using the Trolox equivalent antioxidant capacity assay (TEAC), and both showed significantly greater antioxidant capacity than vitamin C. Conclusions: The GSE/FluorinolReg. combination showed both a significant antiplaque activity and an important antioxidant capacity in vitro, without any bactericidal effects. Significance and Impact of the Study: This is, to our knowledge, the first report on the properties of an original combination of a polyphenolic extract with amine fluoride that could be used for the prevention of oral diseases and oxidative damage associated.