EfTect of growth hormones, genotypes and explants on callus induction in cotton (Gossypium hirsutum L.)

摘要

Seven days old cotyledon and hypocotyl explants of Coker genotypes (Coker 310 and Coker 312) and Indian genotypes (MCU 5 and SVPR 2) were evaluated with different levels of plant growth hormones in MS medium for callus induction. In the present study,the growth hormones viz., IBA, 2,4-D with KT, 2,4-D with TDZ, pIC with KT, pIC with TDZ, NAA with BA, NAA with TDZ and ZT was supplemented to evaluate the callus induction frequency. The best callus induction (85.10 %) was identified from MS medium containing plC and TDZ followed by (83.09 %) from the MS medium supplemented with pIC and KT. Among the genotypes studied, the highest callus induction frequency was exhibited by Coker 310 (79.59 %) and the lowest callus induction was recorded by MCU 5 (61.65 %). Hypocotyl recorded the mean value of 72.34 per cent for the callus induction in different hormonal combinations studied. The mean value of callus induction was 66.56 per cent in cotyledon and it took more time for the callus induction. Hypocotyl was identified as the best explant for the callus induction.