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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Simultaneous separation and on-line concentration of amitrole and benzimidazole pesticides by capillary electrophoresis with a volatile migration buffer applicable to mass spectrometric detection
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Simultaneous separation and on-line concentration of amitrole and benzimidazole pesticides by capillary electrophoresis with a volatile migration buffer applicable to mass spectrometric detection

机译:毛细管电泳同时使用挥发性迁移缓冲液进行毛细管电泳同时分离和在线富集安替利和苯并咪唑类农药,适用于质谱检测

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摘要

This study used capillary electrophoresis (CE) to investigate the simultaneous separation and on-line concentration of five pesticides: amitrole (AMT), carbendazim (MBC), 2-aminobenzimidazole (ABI), thiabendazole (TBZ) and 1,2-diaminobenzene (DAB). A volatile migration buffer was used for the investigation because of the applicability to mass spectrometric (MS) detection. They were separated completely at pH 4.0 as a result of changing pH using formic acid-ammonium formate buffer. Values of the dissociation constant for MBC and DAB estimated from the changes in the mobility with pH showed good agreement with values in the literature. Dissociation constants for AMT and TBZ were estimated. Limits of detection (LODs) for the analytes were on the ppm level with UV detection under the optimized separation condition. On-line concentration by simple stacking mode was not effective except to 2-aminobenzimidazole because of the peak tailing. The addition of formic acid to sample matrix improved the peak shapes. That improvement may be attributed to transient isotachophoretic effect. The concentration factors obtained from the comparison of the LODs were in the range of 7.6-27-fold. This concentration method was applied preliminarily to CE with MS detection. (C) 2004 Elsevier B.V. All rights reserved.
机译:这项研究使用毛细管电泳(CE)来研究五种农药的同时分离和在线浓度:tro胺(AMT),多菌灵(MBC),2-氨基苯并咪唑(ABI),噻苯达唑(TBZ)和1,2-二氨基苯(轻拍)。由于适用于质谱(MS)检测,因此使用了挥发性迁移缓冲液进行研究。通过使用甲酸-甲酸铵缓冲液改变pH值,将它们在pH 4.0下完全分离。由迁移率随pH的变化估算的MBC和DAB的解离常数值与文献中的值显示出很好的一致性。估计了AMT和TBZ的解离常数。在最佳分离条件下,采用UV检测的分析物的检出限(LOD)为ppm级。由于峰拖尾,通过简单堆积模式进行在线浓缩除对2-氨基苯并咪唑外无效。将甲酸添加到样品基质中可改善峰形。这种改善可能归因于瞬时等速电泳效应。从LOD的比较中获得的浓度因子在7.6-27倍的范围内。该浓缩方法已初步应用于具有MS检测的CE。 (C)2004 Elsevier B.V.保留所有权利。

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