ENANTIOMERIC RESOLUTION OF DERIVATIZED DL-AMINO ACIDS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY USING A BETA-CYCLODEXTRIN CHIRAL STATIONARY PHASE - A COMPARISON BETWEEN DERIVATIZATION LABELS

摘要

A chiral stationary phase with immobilized beta-cyclodextrin was used for the liquid chromatographic separation of amino acid derivatives into their enantiomers. Several reagents differing in size, structure and linking group (i.e. the isothiocyanates PITC, NITC, DNITC and DABITC, the activated ester AQC, and the sulfonyl chlorides DNS-Cl and DABS-Cl) were used to derivatize the amino groups of alpha-amino acids prior to separation. A compilation of the selectivity data obtained with the different labels is given as guideline in selecting appropriate labels for a particular separation problem. [References: 31]