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Identification of palmitate-regulated genes in HepG2 cells by applying microarray analysis

机译:应用芯片分析鉴定HepG2细胞中棕榈酸酯调节的基因

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Palmitate is the most abundant saturated fatty acid in the human diet and the major one synthesized de novo. To identify palmitate-regulated genes we performed whole genome mRNA expression profiling by using human hepatoma HepG2 cells. We identified eleven genes which are significantly (single-sided permutational t-test, p < 0.05) regulated by low concentration of palmitate (50 mu M). We observed a decreased expression of five metallothioneins, and an increased expression of liver expressed plasminogen activator inhibitor-1 protein and insulin-like growth factor 11, which play a prominent role in the development of the metabolic syndrome. Comparative promoter analysis in-silico revealed common transcriptional regulation of differentially expressed genes through erythroid kruppel-like factor and members of the zinc binding protein factor family. In conclusion, low physiological palmitate concentrations changed expression of very responsive genes. (C) 2007 Elsevier B.V All rights reserved.
机译:棕榈酸酯是人类饮食中含量最丰富的饱和脂肪酸,并且是从头合成的主要脂肪酸。为了鉴定棕榈酸酯调节的基因,我们通过使用人肝癌HepG2细胞进行了全基因组mRNA表达谱分析。我们确定了11个受低浓度棕榈酸酯(50μM)显着调节的基因(单侧排列t检验,p <0.05)。我们观察到五个金属硫蛋白的表达减少,以及肝脏表达的纤溶酶原激活物抑制剂1蛋白和胰岛素样生长因子11的表达增加,它们在代谢综合征的发展中起着重要作用。计算机中的比较启动子分析揭示了通过类红细胞kruppel样因子和锌结合蛋白因子家族成员共同表达差异表达基因的转录调控。总之,低的生理棕榈酸酯浓度改变了非常敏感的基因的表达。 (C)2007 Elsevier B.V保留所有权利。

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