New proceudres for glycophorin A purification with high yield and high purity

摘要

Glycophorin A (GPA) is the major glycoprotein of the human erythrcyte membrane.It is known to form,in SDS gels as well as in a membrane environment,homodimers,and also heterodimers with the homologous molecule glycophorin B (GPB).It is shown in this report that the propensity of GPA todimerize with GPB predludes satisfactory preparation with high yield of pure GPA using classical techniques including SEC and RPLC.It was demonstrated using multiple angle light scatterig that GPA is eluted from RPLC columns as dimers.A convenient procedure was devised which allowed us to get pure GPA with highyield.This proceudre consists of selectivley blocking GPA-GPB heterodimer formation by selective modification of Cysteine 50 of GPB before RPLC.