Optimized methods for utilizing and analyzing dialkylamine tags in encoded combinatorial chemistry are reported. A previously described LC/fluorescence method has been shortened from over 1 h to 6 min. A novel ion exchange LC/MS method for decoding has been developed which is both fast and rugged. The use of quantitative encoding schemes is described. These new techniques have allowed a simplified, automated sample preparation scheme, and over 300 encoded bead samples can be read in a day.
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