Bioconversion of cholesterol to 4-cholesten-3-one by recombinant Bacillus subtilis expressing choM gene encoding cholesterol oxidase from Mycobacterium neoaurum JC-12

Shao Minglong; Rao Zhiming; Zhang Xian; Xu Meijuan; Yang Taowei; Li Hui; Xu Zhenghong; Yang Shangtian

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Bioconversion of cholesterol to 4-cholesten-3-one by recombinant Bacillus subtilis expressing choM gene encoding cholesterol oxidase from Mycobacterium neoaurum JC-12

机译：表达来自新分枝杆菌JC-12的编码胆固醇氧化酶choM基因的枯草芽孢杆菌将胆固醇生物转化为4-胆甾烯-3-

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BACKGROUNDBacillus subtilis 168 was used to express the cholesterol oxidase (EC 1.1.3.6) genes cloned from Mycobacterium neoaurumJC-12 and the bioconversion of cholesterol to 4-cholesten-3-one was realized.

机译：背景枯草芽孢杆菌168用于表达从新分枝杆菌JC-12克隆的胆固醇氧化酶（EC 1.1.3.6）基因，实现了胆固醇向4-胆甾烯-3-酮的生物转化。

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来源
《Journal of Chemical Technology & Biotechnology》 |2015年第10期|共10页
作者
Shao Minglong; Rao Zhiming; Zhang Xian; Xu Meijuan; Yang Taowei; Li Hui; Xu Zhenghong; Yang Shangtian;
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正文语种 eng
中图分类生物工程学（生物技术）;生物化学过程;
关键词
cholesterol oxidase; cholesterol; 4-cholesten-3-one; recombinant Bacillus subtilis; Mycobacterium neoaurum;

机译：胆固醇氧化酶;胆固醇;4-胆固醇-3-一;重组枯草芽孢杆菌;新分枝杆菌;

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1. Bioconversion of cholesterol to 4-cholesten-3-one by recombinant Bacillus subtilis expressing choM gene encoding cholesterol oxidase from Mycobacterium neoaurum JC-12 [J] . Shao Minglong, Rao Zhiming, Zhang Xian, Journal of Chemical Technology & Biotechnology . 2015,第10期

机译：表达来自新分枝杆菌JC-12的编码胆固醇氧化酶choM基因的枯草芽孢杆菌将胆固醇生物转化为4-胆甾烯-3-
2. Bioconversion of 4-androstene-3,17-dione to androst-l,4-diene-3,17-dione by recombinant Bacillus subtilis expressing ksdd gene encoding 3-ketosteroid-DELTA~1-dehydrogenase from Mycobacterium neoaurum JC-12 [J] . Wenqing Zhang, Minglong Shao, Zhiming Rao, The Journal of Steroid Biochemistry and Molecular Biology . 2013,第3期

机译：表达来自新分枝杆菌JC-12的编码3-酮甾体-DELTA〜1-脱氢酶的ksdd基因的枯草芽孢杆菌将4-雄甾烯-3,17-二酮生物转化为雄甾-1,4-二烯-3,17-二酮
3. Bioconversion of 4-androstene-3,17-dione to androst-l,4-diene-3,17-dione by recombinant Bacillus subtilis expressing ksdd gene encoding 3-ketosteroid-triangle-dehydrogenase from Mycobacterium neoaurum JC-12 [J] . Wenqing Zhang, Minglong Shao, Zhiming Rao, The Journal of Steroid Biochemistry and Molecular Biology . 2013,第Null期

机译：表达来自新分枝杆菌JC-12编码3-酮甾体-三角-脱氢酶的ksdd基因的枯草芽孢杆菌将4-雄甾烯-3,17-二酮生物转化为雄甾-1,4-二烯-3,17-二酮
4. Clone and Expression of High Yield Recombinant Trehalose Synthase in Bacillus subtilis [C] . Jing Su, Chunling Ma, Tengfei Wang, International conference on applied biotechnology . 2014

机译：枯草芽孢杆菌的高产量重组海藻糖合酶的克隆及表达
5. Biochemical Characterization of MmgB, a Gene Encoding a 3-Hydroxybutyryl-CoA Dehydrogenase from Bacillus subtilis 168 and Genetic Evidence for the Methylcitric acid Cycle in Bacillus subtilis 168 [D] . Vegunta, Yogesh. 2011

机译：MmgB的生物化学表征，一种编码枯草芽孢杆菌168的3-羟基丁酰-CoA脱氢酶的基因和枯草芽孢杆菌168中甲基柠檬酸循环的遗传证据
6. Efficient 9α-hydroxy-4-androstene-317-dione production by engineered Bacillus subtilis co-expressing Mycobacterium neoaurum 3-ketosteroid 9α-hydroxylase and B. subtilis glucose 1-dehydrogenase with NADH regeneration [O] . Xian Zhang, Zhiming Rao, Lele Zhang, -1

机译：改造的枯草芽孢杆菌共表达新aucorum 3-ketosteroid9α-羟化酶和枯草芽孢杆菌葡萄糖1-脱氢酶的NADH再生高效生产9α-羟基-4-雄烯-317-二酮
7. 4-cholesten-3-one decreases breast cancer cell viability and alters membrane raft-localized EGFR expression by reducing lipogenesis and enhancing LXR-dependent cholesterol transporters [O] . Josiane Elia, Delphine Carbonnelle, Cédric Logé, 2019

机译：通过还原脂肪生成和增强LXR依赖性胆固醇转运蛋白，4-胆甾烯-3-酮降低乳腺癌细胞活力并改变膜筏局部化的EGFR表达
8. Cloning and Expression of the Bacillus anthracis Protective Antigen Gene in Bacillus subtilis. [R] . Ivins, B. E., Welkos, S. L. 1986

机译：枯草芽孢杆菌保护性抗原基因的克隆与表达。

Bioconversion of cholesterol to 4-cholesten-3-one by recombinant Bacillus subtilis expressing choM gene encoding cholesterol oxidase from Mycobacterium neoaurum JC-12

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