Design and In Vitro Evaluation of Simvastatin-Hydroxyapatite Coatings by an Electrochemical Process on Titanium Surfaces

摘要

The aim of this study was to deposit simvastatih-hydroxyapatite (sim-HA) coatings on titanium surfaces by an electro- chemical process and evaluate osteoprogenitor cell responses to sim-HA-coated surfaces. Simvastatin was prepared onto titanium surfaces with varying concentration (10~(-7), 10~(-6), 10~(-5) and 10~(-4) mol/L). Surface characteristics were performed by FSEM,XRD and FTIR. LC-MS/MS method was used for simvastatin quantification in Sim-HA-coated surfaces. Murine calvaria-derived pre-osteoblastic cell (MC3T3-E1) proliferation, alkaline phosphatase activity (ALP) and osteocalcin release were used to measure osteoblastic activities. FSEM observation showed rod-like HA crystals covered on all surfaces. As drug concentration increased, the crystal diameter decreased. XRD and FTIR observations showed all coatings consisted of HA. LC-MS/MS test showed the simvastatin concentration in 10~(-6) mol/L group was 2.77 × 10~(-7) mol/L/cm~2, while in 10~(-7) mol/L group was 1.89 × 10~(-7) mol/L/cm~2. MC3T3-E1 cells grown on 10-(-7) mol/L and 10~(-6) mol/L Sim-HA surfaces showed increased ALP activity as compared to HA-coated surfaces on day 7 (P < 0.05) while at 10 day, all Sim-HA groups were significantly increased than, HA group (P < 0.05). Two Sim-HA groups showed significant increases in osteocalcin production on 7 10 and 14 day (P < 0.05). It showed that incubation with 10~(-7) mol/L simvastatin enhanced the cell proliferation to a statistically significant extent (P < 0.05) compared with the HA group at day 7. It was concluded that simvastatin was successfully deposited into HA coatings using the electrochemical process and the sim-HA coatings enhanced differentiation of osteoprogenitor cells.