Effects of microrough and hierarchical hybrid microanorough surface implants on osseointegration in ovariectomized rats: A longitudinal in vivo microcomputed tomography evaluation

摘要

This study aimed to evaluate the effects of microrough and hierarchical hybrid microanorough surface implants on osseointegration in ovariectomized rats at different time points. Implants with machined, microrough and hierarchical hybrid microanorough surfaces were inserted into the distal femurs of ovariectomized Sprague-Dawley female rats. At weeks 0, 4, and 12 following implantation, in vivo microcomputed tomography (micro-CT) scanning was used to assess bone microarchitectural changes. After 12 weeks, all the rats were sacrificed, and the femurs with implants were harvested for histological analysis and pull-out test. For the Micro-CT analysis, the trabecular number and the bone volume ratio increased significantly in the microrough group (p < 0.01) and microanorough group (p < 0.01) compared with the machined group. The trabecular separation decreased significantly in the microanorough group (p < 0.01) compared with the other two groups. For the maximum pull-out forces and the bone-implant-contact analyses, significant statistical differences were found among the three groups, with the following sequence: microanorough group > microrough group > machined group. The results indicate that the microrough and the hierarchical hybrid microanorough surfaces of the implant can be beneficial to osseointegration under osteoporotic conditions, and the hierarchical hybrid microanorough surface is more efficient.