Interstitial fluid pressurization plays an important role in cartilage biomechanics and is believed to be a primary mechanism of load support in synovial joints. The objective of this study was to investigate the effects of enzymatic degradation on the interstitial fluid load support mechanism of articular cartilage in unconfined compression. Thirty-seven immature bovine cartilage plugs were tested in unconfined compression before and after enzymatic digestion. The peak fluid load support decreased significantly (p<0.0001) from 84±10% to 53±19% and from 80±10% to 46±21% after 18-hours digestion with 1.0 u/mg-wet-weight and 0.7 u/mg-wet-weight of collagenase, respectively. Treatment with 0.1 u/ml of chondroitinase ABC for 24 hours also significantly reduced the peak fluid load support from 83±12% to 48±16% (p<0.0001). The drop in interstitial fluid load support following enzymatic treatment is believed to result from a decrease in the ratio of tensile to compressive moduli of the solid matrix.
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机译：间质液加压在软骨生物力学中起着重要作用，并且被认为是滑膜关节负荷支持的主要机制。这项研究的目的是研究酶降解对无侧限压迫性软骨间质液负荷支持机制的影响。在酶消化之前和之后，对37个未成熟的牛软骨栓塞进行了无限制压缩测试。在以1.0 u / mg湿重和0.7 u湿重消化18小时后，峰值流体负荷支持从84±10％显着降低（p <0.0001）至53±19％，从80±10％降低至46±21％。 / mg-湿重的胶原酶。用0.1 u / ml软骨素酶ABC处理24小时也将峰值液体负荷支持率从83±12％降低到48±16％（p <0.0001）。酶处理后的间质液负荷支持的下降被认为是由于固体基质的拉伸模量与压缩模量之比降低。
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