The Injectable Nano-Scaled Artificial Bone Promotes the Bone Regeneration and Angiogenesis of Rabbit In Vivo

摘要

This study was designed to evaluate the biocompatibility of the injectable nano-scaled artificial bone (INAB) with rabbit osteoblasts in vivo and the efficacy of the injectable nano-vascularized tissueengineered bone (IVTB) on bone angiogenesis and regeneration for rabbits with fracture. In the current study, bone marrow mesenchymal stem cells (BMSCs) from New Zealand white rabbits were induced to differentiate into osteoblasts which were applied to prepare the injectable nano tissue-engineered bone (INTB) in vitro. Then, the cell viability of the osteoblasts in INTB group was investigated by 3-(4,5-dirnethy1-2-thiazolyl)-2,5-dipheny1-2-H-tetrazolium bromide (MU), followed by alkaline phosphatase (ALP) determination. Meanwhile, the morphology and biocompatibility were observed under microscopes. In vivo, the osteoblasts/endothelial co-cultured cells were first prepared using rabbit renal vascular endothelial cells and osteoblasts. Then IVTB was produced with the co-cultured cells and INAB. General performance, histomorphological assay, blood capillary counting and trabecula area percent were recorded to measure the effects of IVTB on the rabbits with bone defects. Our results indicated that there was no difference of cell viability and ALP value between INAB and control groups in vitro. Moreover, cell morphology was normal like control group and the osteoblasts exhibited good biocompatibility with INAB. In vivo, the rabbits had more angiogenesis and trabecula area percent in IVTB group than those in other groups. These results suggest that INAB has good biocompatibility with rabbit osteoblasts in vitro and IVTB may promote vascularization and bone regeneration in rabbits. Hence, INTB and IVTB might be a better bone repairing material.