Determination of hydrogen peroxide and organic peroxides in micellar and aqueous-organic media using a spectrophotometric biosensor based on horseradish peroxidase

摘要

The expedience of the target-specific control of the properties of a medium (using micellar and aqueous-organic media), in which an indicator process providing the background for the operation of a spectrophotometric sensor based on a polyelectrolyte complex (horseradish peroxidase-chitosan) is conducted, is shown experimentally and proved by calculations of kinetic parameters of the enzymatic reaction. The application of the sensor ensures an increase in the sensitivity of the determination of peroxides of different nature and structures (e.g., hydrogen and urea peroxides, benzoyl peroxide, 2-butanone peroxide and tertbutyl hydroperoxide) in complex matrixes. The proposed approach allows the analyst not only to regulate the performance characteristics of the developed procedures for the determination of peroxides depending on the analytical task, but also to extend the range of test samples (including those insoluble in water) analyzed with no sample preparation.