Evaluation of diatomea algae Thalassiosira weissflogii sensitivity to chloride mercury and methylmercury by chlorophyll fluorescence analysis

摘要

Measurement of chlorophyll fluorescence has been shown to be a rapid, non-invasive, and reliable method to assess photosynthetic performance in a changing environment. In our study, the pulse-amplitude-modulation (PAM)-fluorometric method was used to evaluate the sensitivity to chloride mercury and methylmercury chloride of diatomea microalgae Thalassiosira weissflogii. We found that 10~(-6) and 10~(-7) M MeHg led to a slow decrease in the PS II activity following for prolonged lag phase, whereas the algae was not sensitive to the same concentrations of HgCl_2. However observed PS II inactivation by methylmercury was not complete and about 10 percents of the cells kept the high level of PS II activity as it was shown by microfluorometric analysis. These cells could determine adaptation of algae to methylmercury effect. Both toxicants decreased the rate of PS II reparation, as well as increased a heat pathway of excitation dissipation in PS II antennae complex.