首页> 外文期刊>Biochimica et Biophysica Acta. General Subjects >Hydogen peroxide-dependent photocytotoxicity by phloxine B, a xanthene-type food colorant.
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Hydogen peroxide-dependent photocytotoxicity by phloxine B, a xanthene-type food colorant.

机译:hl吨型食用色素福禄生B依赖于过氧化氢的光细胞毒性。

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BACKGROUND: Phloxine B (PhB; 2',4',5',7'-tetrabromo-4,5,6,7-tetrachloro-fluorescein), an artificial xanthene colorant, has been used as a red coloring agent in drugs and cosmetics as well as foods in some countries. However, little effort has been devoted to the study of this colorant as a potentially useful medicinal agent. METHODS: We investigated the daily light-induced photocytotoxicity of PhB in two human leukemia cells, HL-60 and Jurkat, and its underlying mechanisms by in vitro experiments using antioxidants. REUSLTS AND CONCLUSIONS: PhB inhibited cell proliferation more preferentially to HL-60 cells than to Jurkat cells. Co-treatment of catalase completely blocked the photocytotoxicity by PhB in HL-60 cells, whereas the effect of histidine was only partial, suggesting that hydrogen peroxide (H(2)O(2)), rather than singlet oxygen, might be a prerequisite for the PhB-induced HL-60 cell death. Actually, PhB produced a significant amount of H(2)O(2) in the media as well as in the cells in concentration- and light-dependent manners. Furthermore, methionine, a hypochlorous acid (HOCl) scavenger, also significantly attenuated the cytotoxicity in HL-60 cells, but not in Jurkat cells, indicating the involvement of myeloperoxidase (MPO)-dependent hypohalous acid formation during the photocytotoxicity. In vitro experiments revealed that halogenated tyrosine was generated from the reaction of bovine serum albumin with PhB and HL-60 cell lysate. The present findings suggested that PhB induced a differential photodynamic action in the MPO-containing leukemia cells through an H(2)O(2)-dependent mechanism. GENERAL SIGNIFICANCE: Our findings provide new insights into the molecular mechanisms underlying the PhB-induced apoptosis and also evaluated PhB as a promising PDT agent.
机译:背景:人工x吨着色剂Phoxxine B(PhB; 2',4',5',7'-四溴-4,5,6,7-四氯荧光素)已用作药物中的红色着色剂,化妆品以及某些国家/地区的食品。然而,很少有人致力于研究这种着色剂作为潜在有用的药物。方法:我们通过抗氧化剂的体外实验,研究了光对PhB在两种人类白血病细胞HL-60和Jurkat中的光致光毒性,以及其潜在的机制。结论和结论:PhB对HL-60细胞的抑制作用比对Jurkat细胞的抑制作用更为优先。过氧化氢酶的共同处理完全阻断了PhB在HL-60细胞中的光细胞毒性,而组氨酸的作用只是部分的,表明过氧化氢(H(2)O(2))而不是单线态氧可能是先决条件PhB诱导的HL-60细胞死亡。实际上,PhB以浓度和光依赖的方式在培养基以及细胞中产生了大量的H(2)O(2)。此外,甲硫氨酸,一种次氯酸(HOCl)清除剂,也可显着减弱HL-60细胞的细胞毒性,但不会减弱Jurkat细胞的细胞毒性,这表明在光细胞毒性过程中,涉及过氧化酶(MPO)依赖性次卤酸的形成。体外实验表明,牛血清白蛋白与PhB和HL-60细胞裂解液反应生成卤代酪氨酸。本研究结果表明,PhB通过H(2)O(2)依赖性机制在含MPO的白血病细胞中诱导了不同的光动力作用。一般意义:我们的发现为PhB诱导的细胞凋亡的分子机制提供了新的见解,并评估了PhB是一种有前途的PDT剂。

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