Characterization of a highly Cu~(~(2+)) -selective fluorescent probe derived from rhodamine B

摘要

A rhodamine B derivative was synthesized and characterized as a highly selective and sensitive probe for Cu~(2+) in ethanol-water solution (2:3, v.v, pH7.4, 50 mM HEPES). A prominent fluorescence enhancement at 575 ran was observed in the presence of Cu~(2+), accompanied by the change in the absorption spectrum. Under the optimal conditions, a good linear range of 0.5-1.5 礛 with a detection limit of 1.6 x 10~(-7) M were obtained. Furthermore, confocal laser scanning microscopy experiments have proven that this probe is cell-permeable and can respond to changes in intracellular Cu~(2+) in living cells.