Development of an In Vitro Regeneration System in Sorghum [Sorghum bicolor (L.) Moench] Using Root Transverse Thin Cell Layers (tTCLs)

摘要

An efficient and reproducible plant regeneration system was developed from cells or tissues of agronomically important Indian sorghum genotypes including 2 commercial cultivars (IMSH27 & K8) of Sorghum bicolor (L.) Moench, Callus induction and plant regeneration were achieved on transverse thin cell layers (tTCL) of roots from aseptically germinated 7-day-old seedlings Callus response depended on the genotype, the concentrations and composition of growth substances and number of in vitro regenerationcycles undergone by the donor plant Murashige and Skoog (MS) medium supplemented with 45-181 uM 2,4-dichlorophenoxy acetic acid (2,4-D), 5 4-21 5 pM naphthalene acetic add (NAA), 5 7-22 8 uM indole acetic acid (IAA) and 4.9-19.7 [jM indole butyric acid(IBA), and combined with 10% (v/v) coconut water (CW) was used for callus induction The calli were cultured on MS medium supplemented with 2.2-17,8 uM 6-benzylaminopurine (BAP) combined with 2 3 uM 2,4-D or 2.7 uM NAA Highly efficient differentiations ofmultiple shoot buds were initiated within 4 weeks of culture Root induction was achieved on half-strength MS medium containing IAA (2,9-28,5 uM) Rooted plants were successfully acclimatised, with the survival rate reaching almost 80% These plants grew normally without showing any morphological variation.