Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones

摘要

The present investigation was conducted to study the effects of auxin and cytokinin on callus induction from leaf blade, nodal, and runner segments of 3 strawberry clones. Among the 32 media combinations tested, 4.0 mg/L NAA + 1.5 mg/L IBA yielded the highest percentage of callus in all types of explants. The rate of adventitious shoot regeneration from callus was also influenced by explant type and concentration of BA in MS medium. The highest shoot regeneration rate was observed in leaf derived callus of clone Rabi-01 on media having 3 mg/L BA. Adventitious shoots were rooted, acclimatized, and subsequently transplanted to the field and observed until fruit production.