Hookworm burden reductions in BALB/c mice vaccinated with recombinant Ancylostoma secreted proteins (ASPs) from Ancylostoma duodenale, Ancylostoma caninum and Necator americanus.

摘要

A study was conducted to determine whether Ancylostoma secreted proteins (ASPs) cloned and expressed from different hookworm species will cross protect against Ancylostoma caninum (Ac) larval challenge. Cross-species protection against A. caninum challenge infections was observed in mice with immunizations using ASP-1 from the human hookworms A. duodenale and Necator americanus. The degree of protection was proportional to the extent of amino acid sequence homology between the ASP immunogen used for vaccination and the Ac-ASP-1 produced by the challenge larval strain. Vaccine protection decreased significantly as amino acid sequence homologies diverged 10% or more. Ac-ASP-2, a molecule cloned from A. caninum having 55% amino acid sequence homologyto the C-terminus of Ac-ASP-1, did not elicit vaccine protection.