Investigating the effects of Sweetpotato chlorotic stunt virus (SPCSV) infection to sweetpotato plants using light and elec tron microscopy

摘要

The most important changes due to Sweetpotato chlorotic stunt virus (SPCSV) infection were confined to the vein region. In general, almost all the anatomical characters of the midrib investigated by light microscopy were increased. However, a reduction was observed in the diameter of xylem vessels and phloem area as well as the thickness of the leaf blades. Examination of the leaf epidermis, obtained through scanning electron microscopy (SEM) revealed that the viral infection increased the pearl glands in number as well as its diameter. The dimension of the stomata guard cells was also increased. On the other hand, several cytopathological alterations were also observed through investigating the ultra-thin sections using TEM. Phloem parenchyma showed multivesicular bodies due to increasing the vesiculation rate over the whole cell. Meantime, the chloroplasts contained much more starch grains comparing to those of healthy cells.