首页> 外文期刊>Veterinary Microbiology >Bovine lactoferrin serves as a molecular bridge for internalization of Streptococcus uberis into bovine mammary epithelial cells.
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Bovine lactoferrin serves as a molecular bridge for internalization of Streptococcus uberis into bovine mammary epithelial cells.

机译:牛乳铁蛋白可作为乳房链球菌内化进入牛乳腺上皮细胞的分子桥。

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摘要

Streptococcus uberis, an environmental mastitis pathogen, is an important causative agent of mastitis in dairy cattle throughout the world. Research from our laboratory demonstrated that bovine lactoferrin (LF), a whey protein present in milk and nonlactating cow mammary secretions, significantly enhanced adherence of S. uberis to mammary epithelial cells in culture. Subsequent research from our laboratory identified S. uberis adhesion molecule (SUAM) showing an affinity for LF. The objective of the present investigation was to test the hypothesis that the interaction between SUAM, bovine LF, and a putative LF receptor on the bovine mammary epithelial cell surface could serve as a bridging molecule for internalization of S. uberis into mammary epithelial cells. When internalization assays were conducted using cell growth medium containing bovine LF, a significant increase in internalization of S. uberis into mammary epithelial cells was observed. However, this effect was reversed when assays were conducted in the presence of antibodies to bovine LF suggesting that internalization of S. uberis into mammary epithelial cells, at least in part, was mediated by LF ligands. When S. uberis was pretreated with antibodies to SUAM, internalization in the presence of LF was reduced in the same manner as observed with antibodies to LF. Transmission and scanning electron microscopy results demonstrated that streptavidin-coated gold particles specifically localized on biotinylated LF receptors on S. uberis and mammary epithelial cell surfaces supporting the availability of LF receptors. Collectively, these results suggest that LF serves as a bridging molecule between SUAM located on the surface of S. uberis and LF receptors located on the surface of mammary epithelial cells thus enhancing internalization of S. uberis into host cells. Exploitation of LF as a molecular bridge for internalization of S. uberis into mammary epithelial cells may confer a significant advantage allowing mammary gland infection.
机译:乳房链球菌是环境性乳腺炎的病原体,是全世界奶牛乳腺炎的重要病原体。来自我们实验室的研究表明,牛乳铁蛋白(LF)是存在于牛奶和非泌乳的牛乳腺分泌物中的乳清蛋白,可显着增强 S的粘附性。乳房到培养的乳腺上皮细胞。我们实验室的后续研究确定了 S。对LF具有亲和力的uberis粘附分子(SUAM)。本研究的目的是检验以下假设:牛乳腺上皮细胞表面上SUAM,牛LF和推定的LF受体之间的相互作用可以充当iS内部化的桥接分子。乳房进入乳腺上皮细胞。当使用包含牛LF的细胞生长培养基进行内化测定时,i的内化显着增加。观察到乳房进入乳房上皮细胞。然而,当在针对牛LF的抗体的存在下进行测定时,这种作用被逆转,表明S的内在化。乳房配体至少部分进入乳房上皮细胞。当 S。用SUAM抗体预处理uberis ,以与LF抗体相同的方式减少LF存在下的内在化。透射和扫描电子显微镜结果表明,抗生蛋白链菌素包被的金颗粒特异性地定位在生物上的生物素化的LF受体上。乳房和乳腺上皮细胞表面支持LF受体的可用性。总的来说,这些结果表明LF充当位于S表面上的SUAM之间的桥接分子。位于乳腺上皮细胞表面的乳房和LF受体增强了 S的内在化。乳房进入宿主细胞。利用LF作为内在化iS的分子桥。乳房进入乳腺上皮细胞可能会带来显着的优势,允许乳腺感染。

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