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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >A bicistronic DNA vaccine containing gene of FMDV and bovine IFN- alpha can prime humoral and cellular immune responses of guinea pigs.
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A bicistronic DNA vaccine containing gene of FMDV and bovine IFN- alpha can prime humoral and cellular immune responses of guinea pigs.

机译:含有FMDV和牛IFN-α基因的双顺反子DNA疫苗可以引发豚鼠的体液和细胞免疫反应。

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摘要

This study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine against foot-and-mouth disease virus (FMDV). Genes encoding the P1, 2A, 3C of FMDV 0/China99 and bovine IFN- alpha were cloned into pcDNA3.1 (+) expression vector under CMV promoter and FMDV IRES control, respectively. The recombinant plasmids were administered to guineapigs by intramuscular injection with mono- or bicistronic expression plasmids and aurintricarboxylic acid (ATA). After 2 sequential vaccinations with plasmid DNA, all of guineapigs were challenged with 100 ID50 FMDV 0/China99. Anti-FMDV antibodies were detected by ELISA and neutralization assay, and the phenotyping of T-cell subpopulation in the peripheral blood were performed by flow cytometry. Vaccination of guinea pigs with mono- or bicistronic expression plasmids induced humoral and cellular immune responses to FMDV antigens. In the challenge test, some of the animals were protected against the challenge of a virulent virus. This study demonstrates that the delivery of FMDV antigens via bicistronic vectors is feasible. Further experimentation with bicistronic delivery systems is required for the optimization and refinement of DNA vaccines to effectively prime protective immune responses against foot-and-mouth disease (FMD).
机译:进行这项研究以评估针对口蹄疫病毒(FMDV)的DNA疫苗的免疫原性和保护功效。将编码FMDV 0 / China99的P1、2A,3C和牛IFN-α的基因分别克隆到CMV启动子和FMDV IRES控制下的pcDNA3.1(+)表达载体中。通过肌内注射单或双顺反子表达质粒和金三羧酸(ATA),将重组质粒施用于几内亚。用质粒DNA连续接种2次后,用100 ID50 FMDV 0 / China99攻击所有的几内亚肽。通过ELISA和中和法检测抗FMDV抗体,并通过流式细胞仪进行外周血T细胞亚群的表型分析。用单顺反子或双顺反子表达质粒接种豚鼠可诱导对FMDV抗原的体液和细胞免疫反应。在攻击试验中,保护了一些动物免受强毒病毒的攻击。该研究表明,通过双顺反子载体递送FMDV抗原是可行的。需要对双顺反子传递系统进行进一步的试验,以优化和完善DNA疫苗,以有效引发针对口蹄疫(FMD)的保护性免疫应答。

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