European plum in vitro regeneration from different organs. (Special Issue: Tree micropropagation and tissue culture.)

摘要

Plum (Prunus domestica L) is an important fruit crop in many countries. Several aspects were studied to improve plum in vitro regeneration. Three different media, namely, B5, WPM, and MS, were evaluated for shoot induction from hypocotyls. Use of B5 medium resulted in significantly higher regeneration efficiency compared to other types of media. Thidiazuron (TDZ) was found to be more effective for promotion of shoot induction than 6-benzylaminopurine (BA). Increase of concentration of TDZ did not show an increase in regeneration efficiency. Study was conducted to evaluate shoot induction from epicotyls of stored mature seeds. Shoots were induced from epicotyls of all 13 plum varieties evaluated. However, strong genotype difference exits for shoot development via epicotyls. Full plants were recovered from the shoots induced from epicotyls.