Isolation and biochemical characterization of a gamma-type phospholipase A 2 inhibitor from Crotalus durissus collilineatus snake serum.

摘要

In the present work, we describe the isolation and partial structural and biochemical characterization of the first phospholipase A 2 inhibitor (gammaPLI) from Crotalus durissus collilineatus ( Cdc) snake serum. Initially, the Cdc serum was subjected to a Q-Sepharose ion exchange column, producing six peaks at 280 nm absorbance (Q1-Q6). Subsequently, Q4 fraction was submitted to affinity chromatography with immobilized PLA 2 BnSP-7, a step that resulted in two fractions (NHS-1 and NHS-2). The latter contained the inhibitor, denominated gammaCdcPLI. The molecular mass of gammaCdcPLI, determined by Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF), was 22,340 Da. Partial sequences obtained by Edman degradation and by mass spectrometry (MALDI-TOF/TOF), showed similarity, as expected, to other related inhibitors. Circular dichroism (CD) analysis showed the presence of approximately 22% alpha helices and 29% beta sheets in the protein secondary structure. Additionally, CD studies also indicated no significant changes in the secondary structure of gammaCdcPLI when it is complexed to BpPLA 2-TXI. On the other hand, dynamic light scattering (DLS) assays showed a temperature-dependent oligomerization behavior for this inhibitor. Biochemical analyses showed gammaCdcPLI was able to inhibit the enzymatic, cytotoxic and myotoxic activities of PLA 2s. Structural and functional studies performed on this inhibitor may elucidate the action mechanisms of PLA 2 inhibitors. In addition, we hope this study may contribute to investigating the potential use of these inhibitors for the treatment of snakebite or inflammatory diseases in which PLA 2s may be involved.