Polybitoxins: A group of phospholipases A(2) from the venom of the neotropical social wasp paulistinha (Polybia paulista)

摘要

The neotropical wasp Polybia paulista is very aggressive and endemic in south-east Brazil, where it frequently causes stinging accidents. By using gel filtration on Sephadex G-200, followed by ion-exchange chromatography on DEAE-Cellulose under a pH gradient, a group of four toxins (designated as polybitoxins-I, II, III and IV) presenting phospholipase A(2) (PLA(2)) activities was purified. These toxins are dimeric with mel. wts ranging from 115,000 to 132,000 and formed by different subunits. The four toxins contain very high sugar contents attached to their molecules (22-43% w/w) and presented different values of pH optimum from 7.8 to 9.0; when dissociated, only residual catalytic activities were maintained. The catalytic activities of polybitoxins (from 18 to 771 mu moles/mg per minute) are lower than that of PLA(2) from Apis mellifera venom and hornetin from Vespa basalis. The polybitoxins presented a non-linear steady-state kinetic behavior for the hydrolysis of phosphatidylcholine at pH 7.9, compatible with the negative co-operativity phenomena. All of the polybitoxins were very potent direct hemolysins, especially the polybitoxins-III and IV, which are as potent as the lethal toxin from V. basalis and hornetin from Vespa flavitarsus, respectively; polybitoxin-IV presented hemolytic action 20 times higher than that of PLA(2) from A. mellifera, 17 times higher than that of neutral PLA(2) from Naja nigricolis and about 37 times higher than that of cardiotoxin from Naja naja atra venom. (C) 1998 Elsevier Science Ltd. All rights reserved. [References: 17]