Correlation of chemical acute toxicity between the nematode and the rodent

摘要

The utility of any non-rodent model system for chemical toxicity screening depends on the level of correlation between its responses and toxic reactions in rodents. Toxicity assays in the nematode Caenorhabditis elegans (C. elegans) can be fast and inexpensive; however few studies have been performed comparing toxic responses in the nematode with data on acute rodent toxicity. We assayed the acute toxicity of 21 types of chemicals in different toxicity categories using C. elegans. The nematodes wereexposed to different concentrations of chemicals in 96-well plate for 24 h. The lethality rate was observed at 2, 4, 12 and 24 h, and median lethal concentration (LC_(50)) was calculated by the Probit method. The lethality rate was counted at 1, 8, 16 and 20 h additionally at the concentrations of 10.000, 21.500 and 46.400 mg ml~(-1) to acquire median lethal times (LT_(50)). The results indicated that the chemical pH could affect the C. elegans LC_(50) value. The pH toleration range for C elegans was more than 2.75. Excluding 4 types of acidic chemicals, there were positive correlations between LC_(50)s of C elegans and LD_(50)s of mouse/rat (r > 0.72, p < 0.01) after both 12 h and 24 h exposure. As to the LC50 data following a 24 h exposure in C. elegans, the correlation of C. elegans LC50s vs. rat LD_(50)s (r = 0.885) was greater than the correlation of mouse vs. rat LD50s (r = 0.879), while the correlation of C. elegans LC50s vs. mouse LD_(50)s (r = 0.741) was lower relative to that of mouse vs. rat LD50s. The data were further compared with an In vitro cytotoxicity model utilizing human epidermal keratinocytes (NHK). The data indicate that the correlation of C. elegans LC_(50)s vs. rat LD5Cls was equal to the correlation of mouse vs. rat LD_(50)s(r = 0.879), and was stronger than the correlation of NHK cell IC_(50)s vs. rat LD_(50)s (r = 0.844). In addition, LT_(50) was significantly correlative with the LC_(50) of C. elegans, indicating that both can be utilized as toxic effect index for further study on acute toxicity testing of chemicals. In summary, C. elegans may be a valuable model for predicting chemicals' acute toxicity in rodents.