Utilization of the growth phase of the first follicular wave for bovine oocyte collection improves blastocyst production

摘要

Characteristics of the follicle population and oocyte developmental competence at selected stages of follicular development were studied in cows with the aim to increese embryo production derived from oocytes collected by transvaginal aspiration. In Experiment 1, the growth phase before dominant follicle selection and the low dominant phase during dominant follicle regression were compared. Twenty-four cyclic Holstein cows, 4 to 6 yr of age, were divided into 2 groups. Animals were synchronized using two injections of prostaglandin F2a at 1 1 d intervals, and onset of estrus was determined (Day 0). Using ultrasonography, all follicles were counted and classified. Oocytes were aspirated once on Days 1 through 3 (Group I, n=5) or Days 15 and 16 (Group 2, n=3) of the estms cycle. The experiment was carried out in 3 replicates. In Experiment 2, the growth phase of the first follicular wave before dominant follicle selection was characterized in detail. Twelve cows of the same breed and age were divided into 3 groups. Their first estrus was synchronized as in Experiment 1, and each following estrus was induced using one injection of prostaglandin F2a administered 4 to 6 d after each aspiration performed. The ovaries were examined, and oocytes were collected repeatedly (total of 5 times per cow) on Days 1 (Group 3, n=4), 2 (Group 4, n=4) or 3 (Group 5, n=4) after estrus at 10 d intervals during a 40 d period. Viable oocytes were matured, fertilized and cultured using the standard methods. In Experimem 1, the mean numbers (+-SD) of all follicles and of recovered and viable oocytes per donor were higher in Group I than in Group 2, but only the mean numbers (+-SD) of larger follicles and recovered oocytes were statistically significant (8.0 +- 0.6 and 6.2 +- 0.6.vs. 3.3 +- 0.5 and 2.8 +- 0.2; P< 0.05). In Experiment 2, the percentage of larger follicles out of all visible follicles and the mean numbers (+-SD) of larger follicles per donor were significantly higher (P<0.05) in Groups 4 (75.7 and 9.1 +- 2.7) and 5 (66.3 and 8.5 +- 2.9) when compared to Group 3 (27.9 and 3.8 +- 0.8). The development rate of fertilized oocytes was significantly higher (P<0.05) in Groups 4 (27.8) and 5 (27.5) than in Group 3 (12.8). It can be concluded that it is possible to improve the efficiency of transvaginal aspiration and in vitro embryo production by utilization of the growth phase of the first follicular wave before dominant follicle selection.