Enhanced Plant let Regeneration and In Vitro Root Production in Vetiver [Vetiveria zizanioides (L.) Nash]

摘要

In vitro plantlet regeneration and production of roots in the shoot base explants of vetiver [Vetiveria zizanioides (L.) Nash] was investigated using Murashige and Skoog's (MS) medium supplemented with different types and concentrations of plant growth regulators. Extensive shoot proliferation was maintained at 5 mg L-1 6-benzylaminopurine (BAP). Increasing the concentration of BAP to 20 mg L-1 significantly inhibited the growth of shoots. Root production was greatly enhanced by the addition of either 0.25 mg L-1 naphthaleneacetic acid (NAA) or 20 mg L-1 indole-3-butyric acid (IBA). Roots induced by NAA were short and thick, whereas those induced by IBA were long, thin and fibrous. Supplementation of 0.5 mg L-1 NAA to MS basal medium and further enrichment with 0.5 mg L-1 kinetin (Ki) significantly enhanced the proliferation of roots. However, addition of 0.2 mg L-1 paclobutrazol (PBZ) into the NAA-supplemented MS medium suppressed both shoot and root growth of vetiver.