Genetic clustering of bovine viral diarrhoea viruses in cattle farms:Genetic identification and analysis of viruses directly from cattle sera[Review]

摘要

The herd-specific genetic clustering of bovine viral diarrhoea virus (BVDV) was studied by phylogenetic analysis of 42 sera collected between 1995-97 from persistently infected cattle on 16 farms in Sweden. The viruses were typed by sequencing a part of the 5' untranslated region of the genome, which had been amplified directly from serum by reverse transcription-polymerase chain reaction. All of the viruses were of BVDV I, either BVDV Ia (NADL-like) or BVDV Ib (Osloss-like) genotypes. No relationship was observed between the geographic region of origin and the character of clinical signs and the typing of the BVDV isolates. However, the phylogenetic analysis revealed a strict herd-specific genetic clustering of the virus. In 15 of the 16 herds, animals were infected with a single strain of BVDV characteristic for that herd. Direct nucleotide sequence analysis from serum can therefore be used as a tool for molecular epizootiology of BVDV infections. [References: 21]