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首页> 外文期刊>The Southeast Asian journal of tropical medicine and public health >SEROEPIDEMIOLOGIC SURVEY IN THAILAND OF COXIELLA BURNETII INFECTION IN CATTLE AND CHICKENS AND PRESENCE IN TICKS ATTACHED TO DAIRY CATTLE
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SEROEPIDEMIOLOGIC SURVEY IN THAILAND OF COXIELLA BURNETII INFECTION IN CATTLE AND CHICKENS AND PRESENCE IN TICKS ATTACHED TO DAIRY CATTLE

机译:牛和小鸡布希氏杆菌感染的血清流行病学调查和奶牛的TIC虫感染

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A seroepidemiologic survey of Coxiella burnetii in cattle and chickens in Thailand was carried out using indirect fluorescent antibody test. Nine of the 130 serum samples from cattle were positive for antibodies against C. burnetii, with antibody titers ranging from 32 to 64. Only one of 113 serum samples from chickens was seropositive, with antibody titer of 16. No C. burnetii-sipecific DNA was detected using restriction fragment length polymorphism-nested PCR in spleens of cattle and chickens. However, coxiella DNA was detected in two of 102 engorged Rhipicephalus microplus ticks attached to dairy cattle. These results indicated that infestation of C. burnetii among cattle and chickens is considerably low in Thailand.
机译:使用间接荧光抗体试验对泰国牛和鸡的柯氏杆菌进行了流行病学调查。牛的130份血清样本中有9份针对伯氏梭状芽胞杆菌抗体呈阳性,抗体滴度在32至64之间。鸡的113个血清样本中只有1份是血清阳性的,抗体滴度为16。无伯氏梭状芽孢杆菌特异性DNA使用限制性片段长度多态性巢式PCR检测牛和鸡的脾脏中的Bcl-1。但是,在与奶牛相连的102个饱满的小脑红R中检测到了Coxiella DNA。这些结果表明在泰国牛和鸡中伯氏梭状芽胞杆菌的侵扰相当低。

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