Formulation and optimization of temozolomidenanoparticles by 3 factor 2 level factorial design

摘要

The aim of this study was to investigate the combined influence of 3 independent variables in the preparation oftemozolomide bearing Non-PEGylated and PEGylated nanoparticles by emulsification solvent evaporation method.A 3 factor 2 level design was used to derive a polynomial quadratic model and construct contour plots to predictresponses. The independent variables selected were concentration of drug (A), concentration of PLGA/PEG-PLGA (B),PVA concentration in aqueous phase (C) and sonication time (D) and evaluated for percentage drug entrapment (PDE)and particle size (PS). A 34 factorial design was used with 4 factors (A, B, C and D) at 3 levels and experimental trialswere performed at all 82 possible combinations. In the present work, 28 runs are considered as the preliminary trialsrevealed that on increasing drug concentration from 2.5 to 5 mg the percent drug entrapment increases, but on furtherincreasing the drug concentration (i.e., to 7.5 mg) no significant effect on the percent drug entrapment and particle sizewas observed. The 34 factorial design was used to derive a polynomial quadratic model and construct contour plots topredict responses. Contour plots were constructed to show the effects of A, B, C and D on the PDE and PS.