ELICITATION OF FORSKOLIN IN SUSPENSION CULTURES OF COLEUS FORSKOHLII (WILLD.) BRIQ. USING BACTERIAL ELICITORS

摘要

MS liquid medium supplemented with 3 mg/1 NAA and 1 mg/1 BAP with pH 5.7 was used for suspension cultures. Suspension cultures subjected to biotic elicitors Staphylococcus aureus. Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli and Proteusaureus induced increase in forskolin content. Among all the elicitors tried Bacillus subtilis proved to be the best elicitor at 20% concentration which was 9 times higher than the control.Elicitor-induced accumulation of secondary metabolites received wide acceptance because of its ability to improve the productivity of the plant cell systems significantly (Eliert 1987). Kang et al. (2009) reported higher accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures. The effects of bacterial elicitors on the growth and forskolin content were studied in the suspension cultures of Coleus forskohlii. In vitro production of bioactive forskolin with increased focus towards yield enhancement can provide new means of obtaining large quantity of forskolin in shorter period of time. In the present study an attempt to enhance the yield of forskolin in in vitro using bacterial elicitors has been carried out.