Boronate-containing copolymer grafted on Eupergit C as matrix for affinity chromatography: Isotherms and kinetics study

摘要

Eupergit C, an acrylic polymer support containing oxirane groups, for pseudo affinity adsorption of bioproducts was modified and functionalized by 1,2-ethanedithiol followed by graft copolymerization of 3-acrylamidophenylboronic acid and N,N-dimethylacrylamide. The resin was characterized by elemental analysis and IR spectroscopy and was subjected to biochemical evaluation through batch binding and column chromatography of mucin. Based on equilibrium adsorption data the Langmuir and Freundlich constants were determined 0.04 and 0.84 at pH 8 and 0.19 and 4.53 at pH 8.5, respectively. Subsequently adsorption data were modeled using the pseudo-first-order and pseudo-second-order kinetics equation. It was shown that the pseudo-second-order kinetic equation could best describe adsorption. The generic application of such copolymer grafted for the adsorption and separation of bioproducts is discussed.