Paenibacillus pini sp. nov., a Cellulolytic Bacterium Isolated from the Rhizosphere of Pine Tree

摘要

Strain S22~T, a novel cellulolytic bacterium was isolated from the rhizosphere of pine trees. This isolate was Gram-reaction positive, motile and rods, and formed terminal or subterminal ellipsoidal spores. S22~T represented positive activity for catalase, oxidase, esterase (C4), esterase lipase (C8), β-galactosidase, leucine arylamidase, and hydrolysis of esculin. It contained meso-diaminopimelic acid as the diagnostic dia-- mino acid in the cell-wall. The predominant isoprenoid quinone was menaquinone 7 (MK-7), and the major cellular fatty acids were anteiso-C_(15:0) (52.9%), iso-C_(16:0) (11.3%), and iso-C_(15:0) (10.0%). The DNA G+C content was 43.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that this isolate belonged to the family Paenibacillaceae. S22~T exhibited less than 97.0% 16S rRNA gene similarity with all relative type strains in the genus Paenibacillus, and the most closely related strains were Paenibacillus anaericanus MH21~T and Paenibacillus ginsengisoli Gsoil 1638~T, with equal similarities of 95.8%. This poly-phasic evidence suggested that strain S22~T should be considered a novel species in the genus Paenibacillus, for which the name, Paenibacillus pint sp. nov., is proposed. The type strain is S22~T (= KCTC 13694~T =KACC 14198~T =JCM 16418~T)