A simple protocol for somatic embryogenesis in Rosa hybrida L. cv. apollo.

摘要

A successful regeneration system from in vitro-derived leaves of Rosa hybrida cv. Apollo, via somatic embryogenesis, has been established. The influence of different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and zeatin on the induction of embryogenic calli was evaluated. The highest percentage of embryogenic callus induction (48.3%) was obtained on full-strength MS medium containing 1.0 mg l-1 2,4-D, while at higher concentrations (4.0 or 5.0 mg l-1 2,4-D) no embryogenic calli were observed. The induction of embryogenic calli also occurred on MS media containing 0.5, 1.0, 2.0, or 4.0 mg l-1 naphthaleneacetic acid (NAA). The lowest concentration of NAA (0.5 mg l-1) showed the highest rate of production of embryogenic calli. For maturation, embryogenic calli were transferred onto MS media containing different concentrations of 6-benzyladenine (BA), thidiazuron (TDZ), or abscisic acid (ABA) alone, or in various combinations. The highest rate of embryo maturation (85.0%) was observed on MS medium supplemented with 1.0 mg l-1 ABA alone. Mature embryos showed the highest rate of germination on full-strength MS medium containing 2.0 mg l-1 BA alone. After rooting, well-developed plantlets were maintained under greenhouse conditions. The morphological characteristics and flower colours of somatic embryo-derived plants were the same as the donor parent.