Kitasatospora saccharophila sp. nov. and Kitasatospora kazusanensis sp. nov., isolated from soil and transfer of Streptomyces atroaurantiacus to the genus Kitasatospora as Kitasatospora atroaurantiaca comb. nov.

摘要

A polyphasic study was undertaken to establish the taxonomic positions of two isolates, SK15~T and SK60~T, from soil samples that were found to have morphological and chemical properties consistent with Kitasatospora strains. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strains SK15~T and SK60~T form novel evolutionary lineages within the radiation of the genus Kitasatospora and share the highest 16S rRNA gene sequences similarities with their closest relatives, Kitasatospora setae I AM 15325~T (97.8%) and Kitasatospora mediocidica IAM 15162~T (97.5%), respectively. However, the results of DNA-DNA hybridization experiment and phenotypic data demonstrated that strains SK15~T and SK60~T are distinct from their closest phylogenetic neighbors and other Kitasatospora species. For chemotaxonomic characteristics, the cell-wall peptidoglycan of strains contained both meso- and LL-diaminopimelic acids as the diamino acids, the predominant quinone system was MK-9(H6) and MK-9(H8), whole-cell hydrol-ysates were rich in galactose, mannose and ribose, and the major fatty acids were C_(16:0), anteiso-C_(15:0), iso-C_(15:0) and iso-C_(16:0). On the basis of both phenotypic and phylogenetic evidence, strains SK15~T and SK60~T were assigned to represent two novel species of the genus Kitasatospora, for which the names Kitasatospora saccharophila sp. nov. (type strain SK15~T=JCM 14559~T=KCTC 19566~T) and Kitasatospora kazusanensis sp. nov. (type strain SK60~T=JCM 14560~T=KCTC 19565~T) are proposed. It is also proposed that Streptomyces atroaurantiacus should be transferred to the genus Kitasatospora as Kitasatospora atroaurantiaca comb. nov. (type strain NBRC 14327~T=DSM 41649~T).