alpha-tocopherol (Vitamin E) and Iloprost attenuate reperfusion injury in skeletal muscle ischemia/reperfusion injury.

摘要

BACKGROUND: The aim of this study was to clarify the role of a-tocopherol (vitamin E) and iloprost on skeletal muscle ischemia/reperfusion injury. METHODS: Setting: animal research laboratory of a university hospital. Experimental design: the iliac arteries of the 24 adult Sprague-Dawley rats were clamped and 4 hours of ischemia followed by 1 hour of reperfusion was applied. In an attempt to decrease reperfusion injury, the rats were given either a-tocopherol (n=8), iloprost (n=6) and 8 rats were given normal saline and served as control group (n=8). Measures: blood pH, pO2, pCO2, HCO3, Na, K, creatine kinase (CPK), lactate dehydrogenase (LDH) values were determined at the end of the reperfusion period. Malondialdehyde (MDA), a product of lipid peroxidation, was measured in blood, muscle and lung as an indicator of free radicals. RESULTS: Blood pO2 and HCO3 levels were significantly high (p<0.05); CPK, LDH and MDA levels were significantly low (p<0.05) in both a-tocopherol and iloprost groups when compared to the control group. Similarly, the MDA levels in the gastrocnemius muscle were significantly low in both treatment groups when compared to the controls (p<0.05). There was no significant difference between groups in other parameters. CONCLUSIONS: The results suggest that, both a-tocopherol and iloprost are useful for attenuating oxidative muscle damage occurring after a period of ischemia/ reperfusion.