Aranciamycin anhydride, a new anthracycline-type antibiotic isolated from Streptomyces sp. Tü 6384

摘要

Streptomycetes isolated from the rhizosphere of Norway spruce were grown in submerged culture in various complex media, and extracts prepared from culture filtrates and biomass were screened by HPLC-diode array analysis to detect novel secondary metabolites.Strain Tu 6384 was found to produce a new anthracycline-rype compound that showed a high similarity with aranciamycin, an antibiotic isolated from Streptomyces echinatus, in its UV-visible spectrum. The compound was characterized as aranciamycin anhydride (1). Strain Tu 6384 was isolated from the rhizosphere of Norway spruce collected in Rammert forest near Tubingen, Germany. It was examined for a number of key properties that are known to be of value in streptomycete systematics. Whole-cell hydrolysates of strain Tu 6384 contained LL-diaminopimelic acid, and hexa- and octahydrogenated menaquinones with nine isoprene units were the predominant iso-prenologs. Partial sequencing of the 16S rRNA gene led to a similarity of 99% with Streptomyces prunicolor.