Evaluation of Anthelmintic Efficacy of Punica Granatum on Phosphatases in Gastrothylax indicus

摘要

Gastrothylax indicus was exposed in vitro to ethanolic and aqueous fruit peel extracts(6.25 - 50 mg)of Punica granatum to evaluate its anthelmintic efficacy. Mortality was observed at 1.53± 0.24h and 2.20± 0.21h for ethanolic and aqueous extracts respectively for the parasite at the highest test concentration of the plant extract. Anthelmintic albendazole was observed to cause mortality instantly (0.08±0.01h) at 80 pg/ml. Tegumental enzymes of the parasite ALP, ACP, G-6-pase and ATPase were foundto be suppressed by both the extracts. Enzyme kinetic studies showed non-competitive inhibition for ACP with both the extracts and albendazole whereas for ALP it was non- competitive with ethanolic and mixed type with aqueous extract. Albendazole showedcompetitive inhibition with ALP.