Effect of thawing regime on post-thaw quality of ram semen frozen in tris, citrate and phosphate based diluents.

摘要

To study the optimum thawing protocol for freezing of ram semen, a factorial experiment was carried out using 3 buffers (Tris fructose citric acid egg yolk glycerol - TFCED, phosphate buffer glucose fructose sodium citrate potassium chloride egg yolk glycerol - a synthetic phosphate medium - phos and sodium citrate glucose egg yolk glycerol - SCGEG), 3 thawing temperatures (37, 40 and 50 degrees C) and 2 thawing periods (8 and 15 seconds) for freezing and thawing of semen of 5 Patanwadi rams. Irrespective of diluents used, the optimum-thawing regime was 40 degrees C temperature for 8 seconds in terms of post-thaw motility and acrosome score for French medium straws. Among the 3 diluents used, TFCEG appeared the best for cryopreservation of ram semen.