Comparative effect of auxin analogues on induction of polyhaploids in triticale and triticale x wheat hybrids through wheat x maize system.

摘要

The study was designed to evaluate two auxins, 2,4-D and picloram for their effectiveness for induction of polyhaploids in triticale and triticale x wheat crosses and to standardize their optimum dose. Ten F₁ and parental genotypes pollinated with maize were subjected to in vivo auxin treatment at three concentrations (100, 250 and 400 mg/l) by injecting at the tiller base of the crossed spikes so as to fill the hollow of their uppermost internode. Both auxins resulted in formation of embryo-carrying seeds, though the performance differed significantly for the three parameters of haploid induction at different concentrations. The highest seed formation, embryo formation, and regeneration frequencies in 2,4-D were recorded at 250, 400 and 250 mg/l concentration, respectively, whereas, 400, 400 and 250 mg/l concentration of picloram, respectively, were best for above traits. All the genotypes led to regeneration of haploid plants at 250 mg/l of both the auxins. On the basis of the three haploid induction parameters and overall success rates, the 250 mg/l concentration of both auxins was optimum.