Molecular marker-based detection of Phlb mutation to increase homoeologouspairing in wheat (Triticum aestivum)

摘要

Modern wheat cultivars belong primarily to two allopolyploid species: hexaploid bread wheat [Triticum aestivum L. (2n - 6x =42; AABBDD)] composed of three related genomes (A, B and D) and tetraploid durum wheat [Triticum turgidum L. (2n - Ax - 28; AABB)] with two related genome (A and B). Chromosome pairing in polyploidy bread and durum wheat occurs only between homologous chromosomes resulting in strictly diploid-like pairing and disomic inheritance. The homologous pairing of polyploidy wheat is controlled by Phi (pairing homologous) locus located on long arm of chromosome 5B which suppresses pairing and recombination between homoeologous chromosomes (Riley and Chapman 1958). Such a Phi enforced pairing was essential for the evolution of these wheats (Jauhar 2003). Cytogenetic manipulation of this pairing is also of great interest from standpoint of alien gene transfer into wheat (Sears 1977, Giorgi 1978)