Sequential injection spectrophotometric determination of iron as Fe(II) in multi-vitamin preparations using 1,10-phenanthroline as complexing agent

摘要

A sequential injection analysis (SIA) system is proposed for the determination of iron (II). Fe(II) was determined by SIA based on the reaction between 1,10-phenanthroline and iron (II), yielding an orange-red colour complex with absorption maximum at 512 nm. The method involved aspiration of 187 mul sample/standard zone followed by a zone of a reagent solution containing 140 mul of 7.8 x 10(-4) Mol l(-1) 1,10-phenanthroline into a carrier stream to be stacked inside a holding coil and flow reversed through a reaction coil to a detector. The optimum condition was evaluated and the calibration curve is linear over a range of 0.25 to 5.0 mg l(-1) of Fe(II) with detection limit of 18 mug l(-1). A sample throughput of 40 h(-1) was established. This technique is found to be simple, accurate, reproducible and sensitive. The proposed method was successfully applied for the determination of total iron as Fe(II) in pharmaceutical products (multi-vitamin tablets) and is especially useful for the determination of iron (II) in tablets with lower iron (II) contents. The results were found to be in good agreement with the results obtained by manual UV/Vis spectrophotometry and flame atomic absorption spectrometry (FAAS) and with claimed values by the manufacturers. (C) 2004 Elsevier B.V. All rights reserved.